Histopathology Protocols

• Handling Tissue
• Histochemistry
• Immunohistochemistry
• Miscellaneous

Handling Tissue Protocols

• Adipose Tissue (PDF)
• Freezing and Fixing Tissue (PDF)
• Mouse Embryos (PDF)
• Mouse Necropsy (PDF)
• Organs: Brain, Heart, Spleen, Pancreas, Kidneys, Intestinal Tract (PDF)
• Perfusion of Mice (PDF)
• Skin & Small Biopsies (PDF)
• Tissue Culture Controls (PDF)

Histochemistry Protocols

• Hematoxylin & Eosin (H&E) (PDF)
• Lectin and Sialidase Treatment (PDF)
• Masson's Trichrome (PDF)
• Oil Red O (PDF)
• Periodic Acid Schiff (PAS) (PDF)
• Sirius Red (PDF)

Immunohistochemistry Protocols

• Anti-Neu5Gc (PDF)
• CD31 (PDF)
• CD45: frozen (PDF)
• GFP (PDF)
• IHC: General (PDF)
• IHC: Spleen, frozen sections (PDF)
• IHC: Spleen, paraffin sections (PDF)

Miscellaneous Protocols

• DNA Isolation (PDF)